Induction of c-Ha-ras transcription in rat cells by simian virus 40 large T antigen.
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چکیده
منابع مشابه
Induction of simian virus 40 antigen in BSC1 transformed cells.
Heating to 45 C induced in virus-free clones of simian virus 40 (SV40) transformed BSC(1) cells the synthesis of SV40 viral antigen, as evidenced by immunofluorescence. Up to 3.8% of the cells exhibited viral antigen 72 hr after heating to 45 C for 30 min. Depletion of arginine from the medium of the heated cells enhanced and increased the percentage of cells synthesizing viral antigen to 11%. ...
متن کاملSimian virus 40 large T antigen binds to topoisomerase I.
Binding of simian virus 40 (SV40) large T antigen to human and calf thymus topoisomerase I (topo I) was readily detected by using modified enzyme-linked immunosorbent assays and immunoblots. In addition to WT T antigen, binding could also be readily demonstrated with T antigen fragments from the amino-terminal region as well as with fragments missing this region, but much less so with small t a...
متن کاملSimian virus 40 T-antigen-related cell surface antigen: serological demonstration on simian virus 40-transformed monolayer cells in situ.
Simian virus 40 (SV40)-transformed monolayer cells were analyzed in situ by indirect immunofluorescence microscopy for the postulated cell surface location of SV40 T-antigen-related molecules. With antisera prepared against purified, sodium dodecyl sulfate-denatured SV40 T-antigen, positive surface staining was obtained when the cells had been treated with formaldehyde before immunofluorescence...
متن کاملAssociation of simian virus 40 T antigen with simian virus 40 nucleoprotein complexes.
Viral nucleoprotein complexes were extracted from the nuclei of simian virus 40 (SV40)-infected TC7 cells by low-salt treatment in the absence of detergent, followed by sedimentation on neutral sucrose gradients. Two forms of SV40 nucleoprotein complexes, those containing SV40 replicative intermediate DNA and those containing SV40 (I) DNA, were separated from one another and were found to have ...
متن کاملStoichiometry of large T antigen and pp53 in complexes isolated from simian virus 40-transformed rat cells.
Simian virus 40-transformed cells synthesize high-molecular-weight protein complexes (22 to 30S) that consist of the virus-coded large T antigen (81,500 daltons) and the cellular antigen pp53. These complexes were partially purified from lysates of transformed rat cells by sucrose velocity sedimentation. The stoichiometry of the two proteins in the complex was studied by direct enzyme-linked im...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1987
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.7.1.556